The aim of present work was to develop an accurate, precise, reproducible and economical UV spectrophotometric method for estimation of guaifenesin. This method was based on area under curve of UV spectrum between 268 to 278 nm and validated as per ICH guideline Q2 (R1). The method has followed linearity in the range of 10-70 µg/ml. The value of correlation coefficient was 0.999. Satisfactory values of percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. Results of the recovery studies (96.42 % to 96.88 %) showed accuracy of the method. LOD and LOQ were calculated as 1.69 µg/ml and 5.13 µg/ml, respectively. The developed method can be used for routine estimation of guaifenesin in bulk and tablet dosage forms.
Loading....